Thermo Fisher Scientific CD282 (TLR2) Monoclonal Antibody (TL2.1), eBioscience
인간 TLR2(CD282)를 인식하는 TL2.1 클론 단일클론 항체로, Flow Cytometry, Western Blot, IHC 등 다양한 응용에 사용 가능. 비결합형 형태로 제공되며, PBS(pH 7.2) 용액에 0.09% sodium azide 포함. 면역반응 및 선천면역 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 8 |
| Immunohistochemistry (IHC) | - | 2 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 2 |
| Immunocytochemistry (ICC/IF) | - | 1 |
| Flow Cytometry (Flow) | 2 µg/test | 16 |
| ELISA | - | 2 |
| Immunoprecipitation (IP) | Assay-Dependent | 1 |
| Neutralization (Neu) | - | 10 |
| Functional Assay | - | 11 |
| Inhibition Assays (IA) | - | 5 |
| Miscellaneous PubMed (Misc) | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rabbit |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TL2.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468614 |
Additional Formats
- Alexa Fluor 488 (#53-9922-42)
- APC (#17-9922-42)
- Biotin (#13-9922-82)
- FITC (#11-9922-42)
- Functional Grade (#16-9922-82)
- PE (#12-9922-42)
- Request custom conjugation
Product Specific Information
Description:
The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). TLR family members are type I transmembrane proteins with leucine-rich extracellular domains and cytoplasmic domains homologous to the type I IL-1 receptor. TLR2 and TLR4 act as pattern recognition receptors involved in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and recognizes diverse pathogens. TL2.1, a blocking antibody, is used to study TLR2-mediated cytokine production in human PBMCs. It can immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.
Applications Reported:
Used for flow cytometry, immunoprecipitation, immunoblotting (WB), and immunohistochemistry (frozen and paraffin sections). Also reported for blocking TLR2-mediated cytokine production. Functional Grade TL2.1 (Product #16-9922-82) is recommended for functional assays.
Applications Tested:
Tested by flow cytometry using normal human peripheral blood cells. Recommended ≤ 2 µg/test (100 µL final volume). Optimize antibody concentration per assay.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
TLR2 is a Toll-like receptor that plays a key role in pathogen recognition and activation of innate immunity. It signals via adaptor molecules such as MyD88 and TIRAP to activate kinases and transcription factors including PKCα/β and NF-κB. TLR2 forms heterodimers with TLR1 or TLR6 to recognize bacterial and mycoplasma lipoproteins. It is highly expressed in peripheral blood leukocytes and mediates responses to Gram-positive bacteria and yeast. TLR2 expression is regulated by immune modulators such as LPS, GM-CSF, IL-1, IL-10 (upregulation), and IL-4, IFN-γ, TNF (downregulation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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