Takara PrimeScript RT Master Mix (Perfect Real Time): Generate cDNA for real-time PCR

Overview

• Reverse transcription in just 15 minutes

• Master mix format reduces pipetting steps and the possibility of contamination

• Works efficiently with GC-rich and/or higher-structured RNA templates

• Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis

More Information

Applications

• First-strand cDNA synthesis for real-time RT-PCR (qPCR)

• Two-step real-time RT-PCR

Choose the right PrimeScript Kit for Real-Time RT-PCR:

Product citations

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Tani, H. et al. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947–56 (2012).

Yasuhara, K. et al. Absence of heat shock transcription factor 1 retards the regrowth of atrophied soleus muscle in mice. J. Appl. Physiol. 111, 1142–9 (2011).

Zhan, Y. et al. A novel taspine derivative, HMQ1611, inhibits breast cancer cell growth via estrogen receptor α and EGF receptor signaling pathways. Cancer Prev. Res. (Phila). 5, 864–73 (2012).

Zhang, X. et al. Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice. Proc. Natl. Acad. Sci. U. S. A. 109, 21534–9 (2012).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.