Takara PrimeScript RT Master Mix (Perfect Real Time): Generate cDNA for real-time PCR
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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
RR036B | Takara RR036B PrimeScript™ RT Master Mix (Perfect Real Time), 800 Rxns pk | 재고문의 | pk | 1,328,000원 | 1,460,800원 | |
RR036A | Takara RR036A PrimeScript™ RT Master Mix (Perfect Real Time), 200 Rxns pk | 재고문의 | pk | 369,000원 | 405,900원 |
Overview
Reverse transcription in just 15 minutes
Master mix format reduces pipetting steps and the possibility of contamination
Works efficiently with GC-rich and/or higher-structured RNA templates
Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis
More Information
Applications
First-strand cDNA synthesis for real-time RT-PCR (qPCR)
Two-step real-time RT-PCR
Choose the right PrimeScript Kit for Real-Time RT-PCR:
If you want to.... | Use this.... |
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Make cDNA using a master mix that includes RTase and primers | PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B) |
Make cDNA using a kit that provides RTase and primers as separate components | PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B) |
Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR | PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B) |
Perform 1-step real-time RT-PCR with TB Green | One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B) |
Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells | CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734) |
Product citations
Komori, H. et al. α(1)-Acid glycoprotein up-regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress. J. Biol. Chem. 287, 30688–700 (2012).
Liu, H. et al. Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseudo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats. Drug Metab. Dispos. 40, (2012).
Shimura, Y., Shiraiwa, Y. & Suzuki, I. Characterization of the subdomains in the N-terminal region of histidine kinase Hik33 in the cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 53, 1255–66 (2012).
Tani, H. et al. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947–56 (2012).
Yasuhara, K. et al. Absence of heat shock transcription factor 1 retards the regrowth of atrophied soleus muscle in mice. J. Appl. Physiol. 111, 1142–9 (2011).
Zhan, Y. et al. A novel taspine derivative, HMQ1611, inhibits breast cancer cell growth via estrogen receptor α and EGF receptor signaling pathways. Cancer Prev. Res. (Phila). 5, 864–73 (2012).
Zhang, X. et al. Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice. Proc. Natl. Acad. Sci. U. S. A. 109, 21534–9 (2012).
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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