Takara iMatrix-511 (recombinant laminin-511): pluripotent stem cell culture matrix

Propagation of embryonic stem (ES) cells or induced pluripotent stem (iPS) cells requires a culture substrate that retains pluripotency, a stable karyotype, and expression of pluripotency markers. iMatrix-511 is a recombinant laminin-511 E8 fragment product that can be used as an ES or iPS cell-culture substrate.

Propagation of embryonic stem (ES) cells or induced pluripotent stem (iPS) cells requires a culture substrate that retains pluripotency, a stable karyotype, and expression of pluripotency markers. iMatrix-511 is a recombinant laminin-511 E8 fragment product that can be used as an ES or iPS cell-culture substrate. These recombinant laminin-511 E8 fragments permit maintenance of human ES and iPS cells in xeno-free culture conditions and provide greater adhesive properties than either full-length laminin, vitronectin, or undefined basement membrane reagents such as Matrigel. The iMatrix-511 cell-culture reagent allows stem cell culture in the absence of mouse feeder cells.

Recombinant laminin-511 E8 fragments are truncated, functional forms of laminin composed of the C-terminal regions of the alpha, beta, and gamma chains. Laminin-511 E8 (150 kDa) retains the full capability for binding to alpha 6 beta 1 integrin. Based on research performed at Kyoto University and Osaka University, recombinant laminin-511 E8 fragments are available as iMatrix-511. This product is available through an agreement between Takara Bio Inc. and Nippi, Inc.

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Overview

• Chemically defined stem cell culture substrate for animal-free, xeno-free, feeder-free ES or iPS cell culture

• Provides greater adhesion of human ES and iPS cells than Matrigel or full-length laminin

• Laminin-511 E8 fragments sustain long-term self-renewal of human ES and iPS cells in defined xeno-free media with dissociated cell passaging

• Promotes high expression of pluripotency markers, normal karyotype, and retained ability to differentiate into all three germ layers

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Applications

• Stem cell culture under chemically defined, animal-free, xeno-free conditions

• Expansion culture of ES and iPS cells

Components

iMatrix is available in both lyophilized and liquid formats:

• Cat. # T300: 350 µg recombinant laminin-511 E8 fragments (lyophilized)

• Cat. # T303: 350 µg recombinant laminin-511 E8 fragments (liquid, 0.5 mg/ml)

• Cat. # T304: 1,050 µg recombinant laminin-511 E8 fragments (liquid, 0.5 mg/ml)

Product citations

Ido, H. et al. The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin ?? chains in integrin binding by laminins. J. Biol. Chem. 282, 11144-11154 (2007).

Miyazaki, T. et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3, 1236 (2012).

Taniguchi, Y. et al. The C-terminal region of laminin ?? chains modulates the integrin binding affinities of laminins. J. Biol. Chem. 284, 7820-7831 (2009).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.