Takara In-Fusion Cloning technology for high-throughput, seamless, and rapid cloning

In-Fusion Cloning offers one-step, high-efficiency, and high-throughput cloning for projects that require large numbers of cloning reactions, such as expression screening studies and library construction.

Advancements in recombinant antibody-based therapeutic development highlight a need for streamlined, high-throughput workflows that allow rapid generation and screening of functional clones.

Depending on your own high-throughput needs, you may want to choose kits based on reagent configuration.

In-Fusion Cloning offers one-step, high-efficiency, and high-throughput cloning for projects that require large numbers of cloning reactions, such as expression screening studies and library construction.

Advancements in recombinant antibody-based therapeutic development highlight a need for streamlined, high-throughput workflows that allow rapid generation and screening of functional clones.

In‑Fusion Cloning offers a suitable method for rapid cloning of many constructs, with a workflow that is amenable to automation. In‑Fusion Cloning provides >95% efficiency, increasing the likelihood that the correct clones are obtained after one round of cloning.

Benefits of In‑Fusion Cloning over other methods for high-throughput applications

Unlike the Gibson cloning method, In‑Fusion Cloning does not rely on a low-fidelity polymerase to fill junction regions. This ensures that cloning done with the In-Fusion enzyme mix results in accurate sequences.

Conventional cloning methods (e.g., ligation-based cloning and TOPO cloning) introduce limitations such as extra subcloning steps, lower efficiency, and lack of insert directionality, making these methods incompatible with the needs of high-throughput cloning. In-Fusion Cloning is highly efficient, always directional, and suitable for use on automation platforms, thus eliminating bottlenecks associated with traditional cloning methods.

The In‑Fusion Snap Assembly Master Mix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing custom primers for amplification of the desired sequence using our primer design tool.

The In‑Fusion Snap Assembly Master Mix comes in either 500-μl liquid aliquots (standard format) or prealiquoted, lyophilized in 96-well plates (EcoDry format) which are stable at room temperature, to accommodate robotic liquid handling. See the table below for specific reagent configurations for various kits.

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