Takara PrimeScript RT Reagent Kit (Perfect Real Time): generate cDNA for real-time PCR

Overview

• Allows fast, efficient synthesis of cDNA for real-time PCR. This kit is designed for two-step real-time RT-PCR.

• Includes random 6-mers and oligo dT primer for use as reverse transcription primers. The reaction can be performed using one or a mixture of these two primer types; alternately, gene-specific primers may be used.

• Includes EASY Dilution (for Real Time PCR) when generating reference standards for a qPCR standard curve. This reagent facilitates higher accuracy at lower standard concentrations.

More Information

Applications

• cDNA synthesis for real-time PCR

Choose the right PrimeScript kit for real-time RT-PCR:

Note

A standard curve must be generated for quantitation by real-time PCR. Dilution of total RNA or cDNA is necessary in order to include low concentration samples, which are important for a viable standard curve with an appropriately wide range. However, dilution with water or TE can narrow the range of the curve because nucleic acids are unstable at low concentrations in the presence of dH2O or TE. Use EASY Dilution (for Real Time PCR) provided in this kit for generating accurate reference standards.

Product citations

Zha, X. et al. Lactate Dehydrogenase B Is Critical for Hyperactive mTOR-Mediated Tumorigenesis. Cancer Res. 71, (2011).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.