Takara PrimeScript RT Reagent Kit (Perfect Real Time): generate cDNA for real-time PCR
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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
RR037B | Takara RR037B PrimeScript™ RT Reagent Kit (Perfect Real Time), 800 Rxns pk | 재고문의 | pk | 1,328,000원 | 1,460,800원 | |
RR037A | Takara RR037A PrimeScript™ RT Reagent Kit (Perfect Real Time), 200 Rxns pk | 재고문의 | pk | 369,000원 | 405,900원 |
Overview
Allows fast, efficient synthesis of cDNA for real-time PCR. This kit is designed for two-step real-time RT-PCR.
Includes random 6-mers and oligo dT primer for use as reverse transcription primers. The reaction can be performed using one or a mixture of these two primer types; alternately, gene-specific primers may be used.
Includes EASY Dilution (for Real Time PCR) when generating reference standards for a qPCR standard curve. This reagent facilitates higher accuracy at lower standard concentrations.
More Information
Applications
- cDNA synthesis for real-time PCR
Choose the right PrimeScript kit for real-time RT-PCR:
If you want to.... | Use this.... |
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Make cDNA using a master mix that includes RTase and primers | PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B) |
Make cDNA using a kit that provides RTase and primers as separate components | PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B) |
Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR | PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B) |
Perform 1-step real-time RT-PCR with TB Green | One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B) |
Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells | CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734) |
Note
A standard curve must be generated for quantitation by real-time PCR. Dilution of total RNA or cDNA is necessary in order to include low concentration samples, which are important for a viable standard curve with an appropriately wide range. However, dilution with water or TE can narrow the range of the curve because nucleic acids are unstable at low concentrations in the presence of dH2O or TE. Use EASY Dilution (for Real Time PCR) provided in this kit for generating accurate reference standards.
Product citations
Zha, X. et al. Lactate Dehydrogenase B Is Critical for Hyperactive mTOR-Mediated Tumorigenesis. Cancer Res. 71, (2011).
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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