Thermo Fisher Scientific Phospho-LCK (Tyr505) Monoclonal Antibody (SRRCHA), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• eFluor 660
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
This SRRCHA monoclonal antibody recognizes human and mouse LCK when phosphorylated on tyrosine 505 (Y505). Y505 is a negative-regulatory site phosphorylated by CSK kinase. LCK is expressed in T cells and associates with CD4 and CD8 co-receptors. Upon TCR engagement, LCK phosphorylates CD3 and zeta chains, enabling ZAP-70 binding and activation. Activated ZAP-70 phosphorylates LAT, which recruits proteins such as SLP-76, Grb2, and PLCγ1. Mice lacking LCK expression show T cell developmental and functional defects.

Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by intracellular staining of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol.
Refer to BestProtocols®: Staining Intracellular Antigens for Flow Cytometry.
Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.

Target Information

LCK is a Src-family tyrosine kinase essential for T-cell receptor (TCR) signaling. Upon TCR triggering, LCK phosphorylates ITAM motifs in zeta subunits, allowing ZAP70 binding and activation. Activated LCK and ZAP70 phosphorylate LAT, forming a platform for downstream signaling. LCK localizes mainly to the plasma membrane and partially to the Golgi apparatus, influencing Raf activation under weak TCR stimulation.
LCK contains myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains mediating protein–protein interactions. It also regulates apoptosis induced by various stimuli.
Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Multiple splice variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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