Thermo Fisher Scientific Phospho-LCK (Tyr505) Monoclonal Antibody (SRRCHA), PE, eBioscience
인산화된 LCK (Tyr505)을 특이적으로 인식하는 단클론 항체로, 인간 및 생쥐 시료에서 사용 가능. PE 형광 표지로 유세포분석(Flow Cytometry)에 최적화됨. 친화 크로마토그래피로 정제되었으며, 4°C 암소 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SRRCHA |
| Immunogen | Phospho peptide corresponding to LCK (Tyr505) |
| Conjugate | PE |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762600 |
Additional Formats
Product Specific Information
Description:
This SRRCHA monoclonal antibody recognizes human and mouse LCK when phosphorylated on tyrosine 505 (Y505). Y505 is a negative-regulatory site phosphorylated by CSK kinase. LCK is expressed in T cells and associates with CD4 and CD8 co-receptors. Upon TCR engagement, LCK phosphorylates CD3 and zeta chains, enabling ZAP-70 binding and activation. Activated ZAP-70 phosphorylates LAT, which recruits proteins such as SLP-76, Grb2, and PLCγ1. Mice lacking LCK expression show T cell developmental and functional defects.
Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-diluted and tested by intracellular staining of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol.
Refer to BestProtocols®: Staining Intracellular Antigens for Flow Cytometry.
Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Target Information
LCK is a Src-family tyrosine kinase essential for T-cell receptor (TCR) signaling. Upon TCR triggering, LCK phosphorylates ITAM motifs in zeta subunits, allowing ZAP70 binding and activation. Activated LCK and ZAP70 phosphorylate LAT, forming a platform for downstream signaling. LCK localizes mainly to the plasma membrane and partially to the Golgi apparatus, influencing Raf activation under weak TCR stimulation.
LCK contains myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains mediating protein–protein interactions. It also regulates apoptosis induced by various stimuli.
Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Multiple splice variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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