Thermo Fisher Scientific Phospho-4EBP1 (Thr36, Thr45) Monoclonal Antibody (V3NTY24), PE, eBioscience
인간 및 마우스 4EBP1의 Thr36/Thr45 인산화 형태를 인식하는 단클론 항체. Flow Cytometry용으로 최적화된 PE 형광표지. 세포 내 인산화 단백질 분석에 적합. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific Phospho-4EBP1 (Thr36, Thr45) Monoclonal Antibody (V3NTY24), PE, eBioscience
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 4 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V3NTY24 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572692 |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The V3NTY24 monoclonal antibody recognizes human and mouse eukaryotic translation initiation factor eIF4E-binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46.
4E-BP1 acts as a translation repressor by binding to eIF4E in its non-phosphorylated form. Phosphorylation at multiple sites releases this repression, enabling cap-dependent translation.
FRAP/mTOR phosphorylates Thr37 and Thr46, while ATM phosphorylates Ser111. Phosphorylation at Thr37/Thr46 is inhibited by PI3 kinase inhibitors LY294002 and wortmannin.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. Recommended use: 5 µL (0.06 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).
Recommended Protocols:
- Protocol A: Two-step protocol for intracellular (cytoplasmic) proteins – optimal flexibility.
- Protocol C: Two-step protocol (Fixation/Methanol) – best for phospho-specific discrimination.
- Protocol B: One-step protocol for intracellular (nuclear) proteins – not recommended.
Refer to the BestProtocols® section for detailed procedures.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Target Information
This gene encodes a translation repressor protein that binds eukaryotic translation initiation factor 4E (eIF4E), inhibiting complex formation and translation initiation. Phosphorylation in response to stimuli such as UV or insulin signaling causes dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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