Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CEW33D), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• View 2 publications

Product Specifications

Available Formats

• APC
• Biotin
• Brilliant UV 615
• eFluor 660
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 690
• PE
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

The CEW33D monoclonal antibody reacts with human CD183 (CXCR3), a G protein-coupled chemokine receptor that binds CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC). CD183 is associated with type 1 immunity and is upregulated in Th1 cells, CD8 effector cells, NK, and NKT cells. It mediates infiltration of inflamed tissues and is also expressed on some B cells and plasmacytoid dendritic cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use 5 µL (0.5 µg) per test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test.

Light Sensitivity: Protect from light; tandem dye is photo-sensitive.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These ligands belong to the CXC chemokine subfamily. Binding induces leukocyte trafficking responses such as integrin activation, cytoskeletal changes, and chemotactic migration. The receptor couples with Gi proteins and undergoes phosphorylation-dependent internalization upon activation. It is expressed in effector/memory T cells and inflamed tissues, mediating recruitment of inflammatory cells. This receptor is a potential target for anti-inflammatory drug development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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