Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
View 12 publications

Product Specifications

Available Formats:

• APC
• Biotin
• Brilliant Violet 421 / 480
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S / 690
• PE / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein–linked chemokine receptor binding CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC). CD183 plays a role in CD4 T cell responses to grafts; knockout mice show compromised allograft rejection. Expression is found on NK cells, subsets of T lymphocytes, Tregs, and Th1-polarized cells.

CXCR3-173 affects chemotaxis in response to ligand, and ligand presence eliminates antibody staining. In vivo addition delays cardiac and pancreatic islet allograft rejection.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Light Sensitivity: Protect vial and samples from light.
Fixation: Use IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in dark.
Excitation: 488–561 nm; Emission: 775 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These belong to the CXC chemokine subfamily, characterized by conserved cysteine residues. Chemokine binding induces leukocyte migration, integrin activation, and cytoskeletal changes. Inhibition by Bordetella pertussis toxin indicates Gi protein coupling. Signal transduction may involve pathways common to other chemokine receptors.

Chemokine-induced receptor internalization leads to rapid, transient responses, restored upon recycling. Expressed in effector/memory T cells and inflamed tissues, CXCR3 and its ligands contribute to inflammatory cell recruitment. The receptor is a potential target for small-molecule antagonists for inflammatory disease treatment. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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