Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PE-Cyanine7, eBioscience
마우스 CD170(Siglec F)을 인식하는 단클론 항체로, PE-Cyanine7으로 결합되어 유세포분석에 적합합니다. 친화 크로마토그래피로 정제되었으며 0.2 mg/mL 농도의 액상 형태입니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.125 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1RNM44N |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569/780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802251 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- Brilliant UV 395, 563, 615, 737
- Brilliant Violet 480, 605, 711
- eFluor 660
- NovaFluor Blue 610-70S, 660-120S, 800
- NovaFluor Red 685, 710
- NovaFluor Yellow 690, 810
- PE, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 780
- Request custom conjugation
Product Specific Information
This 1RNM44N monoclonal antibody recognizes mouse CD170 (Siglec F), a cell surface lectin of the Ig superfamily that binds sialic acid–containing glycoconjugates. Siglec F is mainly expressed on eosinophils and alveolar macrophages, with lower expression on immature myelomonocytic cells. It is the functional ortholog of human Siglec 8 but is not expressed on mast cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on thioglycolate-elicited peritoneal macrophages; ≤0.125 µg/test recommended. Adjust cell number empirically (10⁵–10⁸ cells/test).
Light Sensitivity: Protect from light due to tandem dye photo-oxidation.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using:
Excitation: 488–561 nm
Emission: 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
SIGLEC5 is a putative adhesion molecule mediating sialic acid–dependent binding to cells. It binds both α2,3- and α2,6-linked sialic acids. Expression is high in peripheral blood leukocytes, spleen, and bone marrow, and lower in lymph node, lung, appendix, placenta, pancreas, and thymus. It is expressed by monocytes and neutrophils but absent in leukemic cell lines representing early myelomonocytic differentiation stages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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