Thermo Fisher Scientific CD195 (CCR5) Monoclonal Antibody (NP-6G4), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD195 (CCR5) Monoclonal Antibody (NP-6G4), PE-Cyanine7, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 2 publications

Product Specifications

Available Formats

• Alexa Fluor 488
• APC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The NP-6G4 monoclonal antibody reacts with human CD195 (CCR5), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1α, MIP-1β and RANTES.
Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages, and immature dendritic cells. CCR5 functions as a receptor to direct lymphocyte chemotaxis and serves as the primary co-receptor for macrophage-tropic HIV-1, binding through gp120.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells.
Recommended staining: 37°C for 1 hour in Flow Cytometry Staining Buffer.
Use at 5 µL (0.5 µg) per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test.

Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

HIV and related viruses require coreceptors in addition to CD4 for infection. Among the chemokine receptor family, CCR5 acts as a principal coreceptor for macrophage- and dual-tropic HIV-1 strains, facilitating fusion and entry into human white blood cells.
CCR5 is required for infection by HIV-1, HIV-2, and SIV. Beta-chemokines RANTES, MIP-α, and MIP-β bind CCR5 and inhibit M-tropic HIV-1 infection. CCR5 associates with the CD4-gp120 complex, enabling membrane fusion and virus entry. CCR5 mRNA is expressed in lymphoid organs and monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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