Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Functional Grade, eBioscience

Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugates

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 786
• eFluor 450
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 700
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The MIH1 monoclonal antibody reacts with human B7-H1 (PD-L1), a member of the B7 family with a molecular weight of approximately 40 kDa. B7-H1 is expressed on a majority of leukocytes and acts as a ligand for PD-1. The PD-1/PD-L1 interaction inhibits T and B cell responses. MIH1 is reported as a blocking antibody.

Applications Reported:

• Flow cytometric analysis
• Blocking in in vitro functional assays

Applications Tested:

• Tested by flow cytometric analysis of normal human peripheral blood cells
• Effective at ≤1 µg/test (100 µL final volume)
• Recommended to titrate for optimal assay performance

Storage and Handling:

• Use in a sterile environment

Filtration: 0.2 µm post-manufacturing filtered
Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
Aggregation: <10% (HPLC)

Target Information

Programmed death receptor ligand 1 (PD-L1, also known as B7-H1) is a B7 family member that binds to programmed death receptor 1 (PD-1), negatively regulating T-cell receptor signaling. PD-L1 reduces TCR-mediated proliferation and cytokine production, and its expression is abundant in many human and murine cancers, upregulated by IFN-γ stimulation. PD-L1 plays an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.