Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• Biotin
• Brilliant UV 615, 661, 737, 805
• Brilliant Violet 421, 650, 786
• NovaFluor Red 700, 755
• NovaFluor Yellow 690, 700
• PE, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

Description:
The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1), a B7 family member with a predicted molecular weight of ~40 kDa. B7-H1 is expressed on T, B, NK, and dendritic cells, and serves as a ligand for PD-1. The PD-1/PD-L1 interaction inhibits T and B cell responses. MIH5 is a blocking antibody.

Applications Reported:

• Flow cytometric analysis
• Blocking in in vitro and in vivo functional assays

Applications Tested:

• Tested by flow cytometric analysis of mouse splenocytes.
• Recommended ≤0.5 µg/test (in 100 µL final volume).
• Cell number: 10⁵–10⁸ cells/test (empirically determined).
• Optimize titration for best assay performance.

Storage and Handling:

• Use in a sterile environment.
• 0.2 µm post-manufacturing filtered.
• Purity: >90% (SDS-PAGE).
• Endotoxin: <0.001 ng/µg antibody (LAL assay).
• Aggregation: <10% (HPLC).

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds to PD-1, a receptor that negatively regulates T-cell receptor (TCR) signaling. PD-L1 engagement decreases TCR-mediated proliferation and cytokine production. PD-L1 is expressed abundantly in murine and human cancers and is upregulated by IFN-γ, suggesting its role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.