Thermo Fisher Scientific AHR Monoclonal Antibody (FF3399), eBioscience
인간 AHR 단백질을 인식하는 FF3399 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. 0.5 mg/mL 액상 형태, PBS(pH 7.2)에 0.09% sodium azide 포함. 높은 순도(>90%)와 낮은 응집도(<10%)로 연구용에 적합.
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific AHR Monoclonal Antibody (FF3399), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 20 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | - |
| Flow Cytometry (Flow) | Assay-dependent | View 2 publications |
| Immunoelectrophoresis (IE) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FF3399 |
| Immunogen | 21H2-AHR (aa 449-848) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865477 |
Available Conjugated Formats
Product Specific Information
Description:
The FF3399 monoclonal antibody recognizes human aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor mediating toxic responses to environmental contaminants such as PCBs and TCDD. AHR also binds naturally occurring compounds from fruits, vegetables, and metabolites. It is found in the cytoplasm complexed with HSP90, p23, and XAP2/AIP/ARA9. Upon ligand binding, AHR translocates to the nucleus, associates with ARNT, and binds to the consensus DNA sequence GCGTG to regulate genes such as CYP1A1. AHR is expressed in many tissues, with the highest levels in the liver, and is involved in Treg and Th17 cell regulation.
Applications Reported:
- Intracellular staining followed by flow cytometry
- Western blotting
- Immunohistochemistry (FFPE)
- Immunocytochemistry (ICC/IF)
Fluorochrome-conjugated FF3399 is recommended for intracellular flow cytometry.
Applications Tested:
Tested in IHC (FFPE), ICC/IF on fixed and permeabilized human cells, and WB. Optimal concentration ≤20 µg/mL; titration recommended for best results.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
AHR (Ah Receptor) is part of a protein family including ARNT (HIF-1 Beta) and Drosophila PER/SIM proteins. It contains an N-terminal PAS domain (~200 aa). AHR binds specific DNA enhancer sequences to initiate transcription of the cytochrome P-450 (CYP1A1) gene. It functions as a ligand-activated transcription factor regulating responses to aromatic hydrocarbons and xenobiotic-metabolizing enzymes. Ligands include polycyclic and halogenated aromatic compounds such as TCDD. In inactive cells, AHR is cytoplasmic and complexed with HSP90; upon ligand binding, it translocates to the nucleus and forms a heterodimer with ARNT. AHR is associated with conditions such as eosinophilic fasciitis and seborrheic dermatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.