Thermo Fisher Scientific Snail1 Monoclonal Antibody (20C8), eBioscience
Human Snail1 단백질을 인식하는 20C8 클론의 단클론 항체로, Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. Affinity chromatography로 정제되었으며, PBS(pH 7.2) 및 0.09% sodium azide 포함. EMT 연구 및 종양 진행 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 10 publications |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 4 publications |
| ELISA | Assay-Dependent | - |
| Immunoprecipitation (IP) | Assay-Dependent | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20C8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865483 |
Additional Formats
Product Specific Information
Description:
The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug belong to a family of transcription factors responsible for inducing epithelial to mesenchymal transition (EMT) during normal organ development and tumor progression. Snail1 acts as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin.
During embryonic development, Snail1 is localized to the mesoderm, while in adults, expression is inducible and limited to activated fibroblasts and some mesenchymal cells. Overexpression of Snail1 has been observed in carcinomas and fibrosarcomas and may serve as an early marker for EMT.
Applications Reported:
This antibody has been reported for use in IHC-P, ELISA, IP, Western blot, and ICC.
Applications Tested:
Tested by IHC on FFPE human breast cancer tissue using low pH antigen retrieval. Can also be used with high pH retrieval. Recommended ≤5 µg/mL; optimize for specific assays.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Zinc finger protein SNAI1 (SNAIL) is a transcriptional repressor that downregulates ectodermal gene expression and contributes to mesoderm and neural crest formation. SNAI1 is implicated in tumor invasion through E-cadherin repression and induction of EMT. In human breast cancer, SNAI1 and SNAI2 (Slug) expression correlate with metastasis, recurrence, and poor prognosis. Snail1 protein in the stroma may serve as a prognostic marker for colon tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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