Thermo Fisher Scientific Cutaneous Lymphocyte Antigen (CLA) Monoclonal Antibody (HECA-452), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Flow Cytometry (Flow)

0.125 µg/test

-

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

HECA-452

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• eFluor 660
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865479

Product Specific Information

Description: This HECA-452 monoclonal antibody reacts with human Cutaneous Lymphocyte Antigen (CLA). This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation.

The HECA-452 antibody has been reported to inhibit sLex binding to E-selectin; however, this property is still under debate in the literature.

Applications Reported: This HECA-452 antibody has been reported for use in flow cytometric analysis, western blotting, functional assays, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This HECA-452 antibody has been tested by flow cytometric analysis on human PBMCs and by immunohistochemistry on FFPE human tissue. The HECA-452 antibody can be used at less than or equal to 0.125 µg/test for flow cytometry and at less than or equal to 10 µg/mL. The antibody performs optimally with low pH antigen retrieval but high pH antigen retrieval can also be used. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.