Thermo Fisher Scientific Cutaneous Lymphocyte Antigen (CLA) Monoclonal Antibody (HECA-452), eBioscience
인체 CLA 항원을 인식하는 HECA-452 단클론 항체로, 피부 T세포 및 염증 부위 연구에 적합. Flow cytometry, IHC, WB 등 다양한 응용 가능. 높은 순도(>90%)와 안정적 액상 형태로 제공되며, 항원 회복 조건 최적화 가능.
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Thermo Fisher Scientific Cutaneous Lymphocyte Antigen (CLA) Monoclonal Antibody (HECA-452), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| Flow Cytometry (Flow) | 0.125 µg/test |
| Functional Assay (Functional) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HECA-452 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865479 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
This HECA-452 monoclonal antibody reacts with human Cutaneous Lymphocyte Antigen (CLA), a cell surface glycoprotein highly expressed on T cells in the skin. CLA is also found on peripheral T and NK cells, monocytes, neutrophils, and CD1a+ dendritic cells. It recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA binds E-selectin and ELAM-1, playing a role in T lymphocyte infiltration and adhesion at cutaneous inflammation sites.
The HECA-452 antibody may inhibit sLex binding to E-selectin, though this remains debated. It is reported for use in flow cytometry, western blotting, functional assays, and IHC of FFPE sections. Tested on human PBMCs and tissues, optimal performance is achieved with low pH antigen retrieval, though high pH can also be used. Recommended careful titration per assay.
Target Information
Cutaneous Lymphocyte Antigen (CLA) is a glycoprotein primarily expressed on skin T cells. It is also present on certain peripheral T and NK cells, monocytes, neutrophils, and CD1a+ dendritic cells. It recognizes sialofucosylated glycans and sialyl Lewis x (sLex), and mediates adhesion with E-selectin and ELAM-1, facilitating T cell migration to sites of skin inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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