Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), APC, eBioscience

Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (Clone 93), APC, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 23 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32.
CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are low-affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes.
Recommended usage ≤ 0.25 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD16 is a 50–65 kDa cell surface molecule existing in two forms:

• Transmembranous form (NK cells, some T cells)
• Phosphatidylinositol-linked form (granulocytes)

CD16 acts as a low-affinity receptor for IgG (FcR III) and mediates ADCC (antibody-dependent cell-mediated cytotoxicity) by NK cells.

CD32 is a 40 kDa glycoprotein functioning as a low-affinity receptor for IgG (Fc gamma RII).
It mediates endocytosis, secretion activation, cytotoxicity, and immunomodulation.
Expressed by B cells, monocytes, granulocytes, and platelets.
Involved in immune complex phagocytosis and regulation of antibody production.
Genetic variations of CD32 may increase susceptibility to systemic lupus erythematosus (SLE).
Multiple transcript variants encoding different isoforms of CD32 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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