Thermo Fisher Scientific PSA-NCAM Monoclonal Antibody (12E3), eBioscience
인간, 생쥐, 랫드의 PSA-NCAM을 특이적으로 인식하는 단클론 항체입니다. 면역조직화학, 면역세포염색, 웨스턴블롯 등에 사용됩니다. 신경 발달 및 종양 연구에 적합하며, 고순도(>90%)로 제조되었습니다. 4°C 보관, PBS 버퍼(0.09% sodium azide 포함).
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Applications and Tested Dilution
| Application | Tested Dilution / Condition |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Publications: View 8 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12E3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572931 |
| Purity | >90%, determined by SDS-PAGE |
| Aggregation | <10%, determined by HPLC |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The monoclonal antibody 12E3 specifically recognizes polysialylated neural cell adhesion molecule (PSA-NCAM) in human, mouse, and rat.
Polysialic acid is a long homopolymer of sialic acid that is negatively charged and attached to neural cell adhesion molecule (CD56), regulating NCAM function. PSA attachment to NCAM inhibits neural cell adhesion.
PSA-NCAM expression is highly regulated and corresponds to specific neural developmental windows involving neural precursor migration and axonal sprouting, guidance, and targeting.
In adults, expression is restricted to regions undergoing self-renewal or exhibiting plasticity such as the olfactory bulb, suprachiasmatic nucleus, hippocampus, hypothalamus, and certain spinal cord nuclei.
PSA-NCAM is re-expressed during tumorigenesis and is found in neuroblastoma and pheochromocytoma cell lines.
Applications Reported:
- Immunohistochemical staining (frozen and paraffin-embedded tissues)
- Microscopy
- Immunocytochemistry
Applications Tested:
Tested by immunocytochemistry of formaldehyde-fixed PC-12 (rat) cells.
Recommended concentration ≤ 20 µg/mL. Optimize titration for best assay performance.
Target Information
Polysialic acid, a negatively charged homopolymer of sialic acid, is attached to NCAM (CD56) and regulates its function.
PSA attachment inhibits neural cell adhesion and is involved in neural development processes such as migration, axonal sprouting, and targeting.
In adults, PSA-NCAM expression is limited to brain regions with neuroplasticity.
It is re-expressed during tumorigenesis and present in neuroblastoma and pheochromocytoma cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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