Thermo Fisher Scientific PCDH1 (Protocadherin 1) Monoclonal Antibody (CSTEM31), eBioscience

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Flow Cytometry (Flow)

0.5 µg/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

CSTEM31

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with proprietary stabilizer

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2865355

Product Specific Information

This monoclonal antibody CSTEM31 reacts with human Protocadherin-1, also known as PCDH1 and cadherin-like protein 1.

Applications Reported: This CSTEM31 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. (Fluorochrome-conjugated CSTEM31 is recommended for use in flow cytometery.)

Applications Tested: This CSTEM31 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human iPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.5 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Target Information

Protocadherin 1, also known as PCDH1 and cadherin-like protein 1, is a transmembrane protein involved in cell-cell adhesion. Protocadherin 1 belongs to a group of cadherin-like proteins termed delta1-protocadherins, which consists of protocadherin-1, -7, -9, and -11(X/Y). The delta1-protocadherins have seven extracellular cadherin-repeats, a transmembrane domain, and a cytoplasmic domain, and can be expressed in two isoforms, short or long. Expression of protocadherin 1 is regulated both temporally and spatially, and plays a role both in early development and mature tissues. In mice, it is expressed in a number of tissues and organs, including the brain, spinal cord, peripheral ganglia, inner ear, hair follicles, kidney, reproductive organs, prostate, and the seminal gland. Notably, protocadherin 1 is a susceptibility gene for Bronchial Hyperresponsiveness in humans. Expression of protocadherin 1 has also been identified on human pluripotent stem cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.