Thermo Fisher Scientific Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (10 µg)/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Rhesus monkey

Published species

Human, Rhesus monkey

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572937

Product Specific Information

Description: The Rhesus Fc Receptor Binding Inhibitor can be used to inhibit the non-specific Fc receptor (FcR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of rhesus tissue. FcR are expressed at varying levels in multiple cell lineages including high expression in myeloid cells, granulocytes, and B cells. The biological function of the FcR, including initiation of endocytosis, phagocytosis, and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to rhesus FcR varies depending on the isotype of the monoclonal antibody.

eBioscience testing indicates that mouse IgG2a isotype is blocked by Rhesus Fc Receptor Binding Inhibitor.

Applications Reported: Rhesus Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis.

To inhibit the non-specific binding of mouse monoclonal antibodies add the Rhesus Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with primary antibody.

Applications Tested: Rhesus Fc Receptor Binding Inhibitor has been pre-titrated and tested by flow cytometric analysis of normal rhesus peripheral blood cells. This can be used at 5 µL (10 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.