Thermo Fisher Scientific Phospho-MECP2 (Ser421) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects a 75 kDa protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment.

Target Information

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3, and MBD4) that contain a methyl-CpG binding domain. MECP2 binds specifically to methylated DNA, a key modification in eukaryotic genomes crucial for mammalian development. It represses transcription from methylated gene promoters and, unlike other MBD family members, is X-linked and subject to X inactivation. MECP2 is dispensable in stem cells but essential for embryonic development. Mutations in the MECP2 gene are the primary cause of Rett syndrome, a progressive neurological developmental disorder and one of the most common causes of intellectual disability in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.