Thermo Fisher Scientific Phospho-LIMK1/LIMK2 (Thr508, Thr505) Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 Rabbit Polyclonal 항체로, LIMK1/LIMK2의 Thr508/Thr505 인산화 형태를 검출. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 -20°C 보관, 연구용으로만 사용.
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Applications
- Western Blot (WB): Tested dilution 1:250
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LIMK1 (Thr-508) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding threonine 508 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Thr-505 in human LIMK2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942943 |
Product Specific Information
The antibody detects a 72 kDa* protein corresponding to the molecular mass of LIMK1 on SDS-PAGE immunoblots of activated mouse recombinant LIMK1, but does not detect this protein after lambda phosphatase treatment.
Target Information
Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified.
LIMK-1 regulates the stabilization of F-actin structures and cofilin activity, indicating its role in signaling pathways related to cell motility and morphogenesis.
LIMK-1 inhibits neuronal differentiation of PC12 cells, acting downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest differing functions during development.
A truncated form of LIMK-2 has been identified in adult testis, thought to arise from an alternative initiation exon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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