Thermo Fisher Scientific Phospho-MECP2 (Ser80) Polyclonal Antibody
Phospho-MECP2 (Ser80) 항체로 인간, 생쥐, 랫드 시료에서 인산화된 MeCP2 단백질 검출에 적합. Western blot, IHC, ICC/IF에 사용 가능. 고순도 친화 크로마토그래피 정제, 안정한 PBS/BSA/glycerol buffer 구성. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | phospho-MeCP2 (Ser-80) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 80 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942944 |
Product Specific Information
The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment.
Target Information
MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3, and MBD4) that have a methyl-CpG binding domain. MECP2 binds specifically to methylated DNA, a key modification in eukaryotic genomes essential for mammalian development. It can repress transcription from methylated gene promoters. Unlike other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensable in stem cells but essential for embryonic development. Mutations in the MECP2 gene cause most cases of Rett syndrome, a progressive neurological developmental disorder and a common cause of mental retardation in females.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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