Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant UV 805
• eFluor 450
• eFluor 506
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation available

Product Specific Information

The 3.9 monoclonal antibody reacts with human CD11c, a 150 kDa integrin alphaX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK cells, dendritic cells, and subsets of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion interactions.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemical staining of frozen tissue sections

Applications Tested:

• Flow cytometric analysis of normal human peripheral blood cells
• Recommended ≤1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
• Antibody should be titrated for optimal assay performance

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD11, together with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.