Thermo Fisher Scientific Mature Macrophage Marker Monoclonal Antibody (eBio25F9 (25F9)), eBioscience
성숙 대식세포 표지 단백질을 인식하는 단일클론 항체로, 인간 시료에 반응합니다. Flow Cytometry, Western Blot, IHC 등 다양한 분석에 사용 가능합니다. 0.25 µg/test 권장, 액상 형태이며 4°C 보관. 연구용으로만 사용됩니다.
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카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Mature Macrophage Marker Monoclonal Antibody (eBio25F9 (25F9)), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 0.25 µg/test | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio25F9 (25F9) |
| Conjugate | Unconjugated |
| Available Formats | Biotin, eFluor 660, Custom conjugation available |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864824 |
Product Specific Information
The monoclonal antibody 25F9 recognizes a protein expressed on mature macrophages, both on the cell surface and within intracellular vesicular structures.
Expression correlates with endocytic macrophages and is useful in various disease models.
No expression is found on immature macrophages, monocytes, or other hematopoietic cells.
Applications Reported
This antibody has been reported for use in:
- Flow cytometric analysis
- Immunoprecipitation
- Western blotting (WB)
- Immunohistology staining of frozen and paraffin-embedded tissue sections
Applications Tested
Tested by flow cytometric analysis of human macrophages.
Recommended usage: ≤ 0.25 µg per test.
A test is defined as the amount of antibody that stains a cell sample in a 100 µL final volume.
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Antibody titration is recommended for optimal assay performance.
Additional Quality Information
- Purity: >90% (SDS-PAGE)
- Aggregation: <10% (HPLC)
- Filtration: 0.2 µm post-manufacturing filtered
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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