Thermo Fisher Scientific CD11a (LFA-1alpha) Monoclonal Antibody (HI111), eBioscience
인간 CD11a(LFA-1α)에 특이적인 HI111 단클론 항체로, Flow Cytometry 및 IHC(Frozen) 등에 사용 가능. 비결합형 포맷으로 제공되며, 0.5 mg/mL 농도의 PBS 용액에 나트륨 아지드 포함. 림프구 부착 및 기능 분석 연구용으로 적합.
- 카탈로그번호
- 14-0119-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD11a (LFA-1alpha) Monoclonal Antibody (HI111), eBioscience
Thermo Fisher Scientific CD11a (LFA-1α) Monoclonal Antibody (HI111), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 3 publications |
| Neutralization (Neu) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467121 |
Additional Formats Available:
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Yellow 660
- NovaFluor Yellow 755
- Custom conjugation available upon request
Product Specific Information
The HI111 monoclonal antibody reacts with human CD11a, a 180 kDa integrin alphaL, also known as lymphocyte function-associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta2), is an adhesion molecule critical for lymphocyte and granulocyte function. Its ligands include CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3). CD11a is expressed by all leukocytes.
Applications Reported:
- Flow cytometric analysis
- Immunohistology staining of frozen tissue sections
- Blocking of CD11a function in mixed lymphocyte reactions (Functional Grade purified HI111 recommended, Product #16-0119)
Applications Tested:
- Tested by flow cytometric analysis of normal human peripheral blood cells
- Effective at ≤1 µg/test (100 µL final volume)
- Cell number range: 10⁵–10⁸ cells/test
- Titrate antibody for optimal performance
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD11a (LFA-1 alpha, ITGAL) and CD18 together form LFA-1, the αLβ2 integrin. CD11a is crucial for leukocyte adhesion and costimulatory signaling via ICAMs 1–3. It exists in low-affinity conformation on leukocytes and shifts to high-affinity upon stimulation, enabling tight ligand binding.
CD11a is involved in immune cell interactions, tissue-specific localization, differentiation, proliferation, and T-cell effector functions. Blocking CD11a is a therapeutic approach in inflammatory diseases; for example, Efalizumab (Raptiva) inhibits T-cell migration, and simvastatin binding to CD11a modulates immune activity. Two transcript variants encoding different isoforms exist. Associated diseases include benign cephalic histiocytosis and leukocyte adhesion deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.