Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-30S, 660-40S, 690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Custom conjugation available upon request

Product Specific Information

Description:
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX). CD11c associates with beta2 integrin (CD18) to form the CD11c/CD18 heterodimer. It is expressed by dendritic cells, subsets of intestinal intraepithelial lymphocytes, and some activated T cells. CD11c/CD18 binds CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion. N418 binds CD11c on splenic dendritic cells in T-dependent spleen areas and precipitates a 150/90 kDa heterodimer.

Applications Reported:
Flow cytometry, immunoprecipitation, and immunohistochemistry (frozen sections).

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize concentration empirically for best results.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD11 proteins (CD11a, CD11b, CD11c) form heterodimeric adhesion molecules with CD18.

• CD11a: Expressed on B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Found on monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed on monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.