Thermo Fisher Scientific Cardiac Troponin I Chimeric Recombinant Mouse Monoclonal Antibody (scFv 180)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.001 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, lambda

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

scFv 180

Immunogen

Cardiac Troponin I peptide (KISASRKLQLKT). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cardiac Troponin I,uniProtId:P19429-1,ncbiNodeId:9606,antigenRange:40-51,antigenLength:210,antigenImageFileName:MA5-47955_Cardiac_Troponin_I_P19429-1_House_mouse.svg,antigenImageFileNamePDP:MA5-47955_Cardiac_Troponin_I_P19429-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074399

Product Specific Information

Specificity: This antibody binds specificially to the cardiac Troponin I Peptide KISASRKLQLKT. This epitope is 100% conserved among a great variety of mammalian species, including most monkeys, Squirrel, Hamster, Mouse, Rat, Rabbit, Whales, Mole-Rat, Antelope and several other mammals.

Target Information

Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.