Thermo Fisher Scientific CD150 Monoclonal Antibody (mShad150), Super Bright 645, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.5 µg/test
  View 3 publications

Product Specifications

Additional Formats Available

• APC
• APC-eFluor 780
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The mShad150 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T cells (particularly Th1) and B cells, with expression rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen.

Signaling through SLAM in T cells induces proliferation and enhances interferon-gamma response. SLAM also plays a role in adhesion between T cells and antigen-presenting cells. The mShad150 antibody stains the CD150⁺CD244⁻CD48⁻CD41⁻ population of pluripotent hematopoietic stem cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorochrome Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) with emission at 645 nm. Recommended filter: 660/20 bandpass. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal signal loss.

Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD150 (SLAM, Signaling Lymphocyte Activation Molecule)
CD150 belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and some B cells, and is rapidly induced on naive T cells after activation. Activated B cells express membrane-bound, soluble, and cytoplasmic isoforms of SLAM.

SLAM-SLAM binding enhances B cell activation and differentiation. The extracellular domain of CD150 serves as the receptor for measles virus and acts as a co-activator for T and B cells. CD150 interacts with SH2D1A and PTPN11 via its cytoplasmic domain. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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