Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), Super Bright 645, eBioscience
Mouse CD127 (IL-7 receptor α) 인식하는 Rat 모노클로날 항체 A7R34 클론. Super Bright 645 형광체로 결합되어 Flow Cytometry에 최적. IL-7 결합 차단 기능으로 T/B 세포 발달 연구에 활용. 광민감성 주의 및 4°C 보관 권장.
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Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), Super Bright 645, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 1.0 µg/test | View 9 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A7R34 |
| Conjugate | Super Bright™ 645 |
| Excitation / Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2744867 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- NovaFluor Red 700
- NovaFluor Red 710
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Request custom conjugation
Product Specific Information
The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the IL-7 receptor.
CD127 is expressed by immature B cells, double-negative thymocytes (CD4-CD8-), single-positive thymocytes (CD4+ or CD8+), but not double-positive (CD4+CD8+) thymocytes. Mature peripheral T cells express CD127 at low levels.
A7R34 inhibits IL-7 binding to its receptor and is used in vivo and in vitro to study IL-7’s role in T/B cell development and activation. Binding of A7R34 blocks SB/199 antibody binding to mouse CD127.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; ≤1 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize by titration.
Super Bright 645 is a tandem dye excited by violet laser (405 nm) and emits at 645 nm. Recommended filter: 660/20 bandpass. Ensure instrument compatibility.
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal impact on brightness or FRET efficiency. Clone-specific performance should be verified empirically.
Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD127 (Interleukin-7 receptor alpha, IL7Rα) is a glycoprotein regulating lymphopoiesis. The active receptor is an alpha/gamma chain heterodimer.
- Gamma(c) chain activates IL7R signal transduction.
- Alpha chain mediates specific signaling.
CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+/CD8+ T cells.
Dysfunction is associated with severe combined immunodeficiency (SCID) forms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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