Takara SMARTer universal low input RNA kit—cDNA synthesis for NGS from degraded samples

Overview

• Sensitivity for analysis of low-input samples—works with 200 pg to 10 ng of rRNA-depleted input (derived from 2–100 ng of total RNA)
• Efficient results from compromised samples—an ideal solution for FFPE samples
• High quality RNA-seq data—consistent RNA-seq data across the recommended input range (200 pg–10 ng)
  • Mappability >80%
  • Reproducibility >0.98
  • ERCC correlation >0.99
  • Number of genes >15,000
• Kit includes both RT and PCR enzymes

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More Information

Applications

• Generate cDNA for RNA-seq from very low amounts of RNA (200 pg–10 ng) that:
  • Is compromised (e.g., obtained from FFPE or LCM samples)
  • Has low RIN values (<8)
  • Is not polyadenylated

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.