Thermo Fisher Scientific Phospho-RHOA (Ser188) Polyclonal Antibody
RhoA 단백질의 Ser188 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC(Paraffin)에서 사용 가능. Human, Mouse, Rat 반응성. PBS buffer, 50% glycerol 보존, -20°C 보관. 연구용 전용.
- 카탈로그번호
- PA5105763
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RHOA (Accession P61586), corresponding to amino acid residues around phosphorylated Ser188. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817162 |
Product Specific Information
This antibody detects endogenous levels of RhoA only when phosphorylated at Ser188.
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in microtubule-dependent signaling required for myosin contractile ring formation during cytokinesis and plays an essential role in cleavage furrow formation.
RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion and serves as a target for the yopT cysteine peptidase from Yersinia pestis and Yersinia pseudotuberculosis.
It stimulates PKN2 kinase activity and may activate PLCE1. RhoA is activated by ARHGEF2, promoting GDP-GTP exchange, and is essential for SPATA13-mediated regulation of cell migration and adhesion dynamics.
The MEMO1-RHOA-DIAPH1 signaling pathway contributes to ERBB2-dependent stabilization of microtubules at the cell cortex, controlling localization of APC and CLASP2 to the cell membrane via GSK3B regulation. Membrane-bound APC allows MACF1 localization required for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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