Thermo Fisher Scientific TWIST1 Monoclonal Antibody (10E4E6)
TWIST1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF, Flow cytometry, ELISA에 적합. Human 및 Mouse 반응성. Protein G 정제, 1 mg/mL 농도의 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10E4E6 |
| Immunogen | Purified recombinant fragment of human TWIST1 (aa: 9–74) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898564 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
TWIST1 acts as a transcriptional regulator. It inhibits myogenesis by sequestering E proteins, inhibiting trans-activation by MEF2, and preventing DNA-binding by MYOD1 through physical interaction. TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B. It regulates cranial suture patterning and fusion, with gene expression varying depending on dimer composition. Mutations in TWIST1 are associated with Saethre-Chotzen syndrome, characterized by coronal synostosis, brachycephaly, low front hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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