Thermo Fisher Scientific UFD1L Monoclonal Antibody (2A6F3)
UFD1L 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. 1 mg/mL 농도의 액상 형태이며, Protein G로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2A6F3 |
| Immunogen | Purified recombinant fragment of human UFD1L (aa: 208–307) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898565 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Ubiquitin-mediated proteolysis requires the transfer of ubiquitin (Ub) to lysine residues on target proteins, leading to their degradation by the 26S proteasome. Ub-fusions are processed by Ub-specific proteases (UBps) or by the Ub-fusion degradation (UFD) pathway. The UFD1 protein, first identified in S. cerevisiae, has a human homolog known as UFD1L. UFD1 attenuates the degradation of Ub-fusions containing amino acid substitutions at the Gly76 moiety by promoting selective multiubiquitination. Mutations in the UFD1 gene are linked to CATCH22 syndrome, characterized by cardiac defects, cleft palate, and hypocalcemia, indicating a role for this pathway in developmental processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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