Thermo Fisher Scientific Placental Alkaline Phosphatase Monoclonal Antibody (3E5)
인간 ALPP 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, ELISA에 적합합니다. 약 58kDa의 ALPP를 검출하며, 단기 4°C, 장기 -20°C 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E5 |
| Immunogen | Purified recombinant fragment of human ALPP expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10980082 |
Product Specific Information
MA5-15652 targets ALPP in indirect ELISA, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human ALPP expressed in E. coli.
MA5-15652 detects ALPP with a predicted molecular weight of approximately 58 kDa.
Target Information
Placental Alkaline Phosphatase (PLAP) plays an important role in the regulation of specific inflammatory disease processes. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney types.
PLAP reacts with a membrane-bound isoenzyme (Regan and Nagao type) occurring in the placenta during the third trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
A proposed function of PLAP is matrix mineralization; however, mice lacking a functional form of this enzyme show normal skeletal development. PLAP has been linked to hypophosphatasia, a disorder characterized by hypercalcemia and skeletal defects. The severity of hypophosphatasia varies depending on the specific mutation, which determines the age of onset and symptom severity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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