Thermo Fisher Scientific Cdc27 Monoclonal Antibody (5C12)
Human CDC27 단백질을 인식하는 Mouse monoclonal antibody(5C12)로, WB, IHC(P), ELISA에 적합합니다. E. coli에서 발현된 재조합 CDC27 단편을 면역원으로 사용하였으며, 91kDa 단백질 검출에 유용합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C12 |
| Immunogen | Purified recombinant fragment of human CDC27 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10979353 |
Product Specific Information
MA5-15655 targets CDC27 in indirect ELISA, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human CDC27 expressed in E. coli.
MA5-15655 detects CDC27, which has a predicted molecular weight of approximately 91 kDa.
Target Information
The CDC27 protein shares strong similarity with Saccharomyces cerevisiae Cdc27 and Schizosaccharomyces pombe nuc2.
It is a component of the anaphase-promoting complex (APC), composed of eight conserved subunits in eukaryotic cells.
APC catalyzes the formation of cyclin B–ubiquitin conjugates responsible for ubiquitin-mediated proteolysis of B-type cyclins.
CDC27 and other APC components contain TPR (tetratricopeptide repeat) domains, important for protein–protein interactions.
This protein interacts with mitotic checkpoint proteins including Mad2, p55CDC, and BUBR1, and may regulate mitotic timing.
Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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