Thermo Fisher Scientific Phospho-USP28 (Ser67) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of USP28 only when phosphorylated at Ser67.

Target Information

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity.

An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families:

1. Ubiquitin C-terminal hydrolases
2. Ubiquitin-specific processing proteases (USPs)
3. OTU-domain ubiquitin-aldehyde-binding proteins
4. Jab1/Pad1/MPN-domain-containing metallo-enzymes

Among these, USPs represent the most widespread and conserved deubiquitinating enzymes across evolution. They tend to release ubiquitin from conjugated proteins and possess conserved catalytic domains containing Cys and His boxes, with variable terminal extensions involved in substrate recognition, localization, and protein interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.