Thermo Fisher Scientific Phospho-USP28 (Ser714) Polyclonal Antibody
USP28 단백질의 Ser714 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 사용 가능. 고순도 정제 및 PBS/glycerol 저장용액 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human USP28 (Accession Q96RU2), corresponding to amino acid residues around phosphorylated Ser714 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817453 |
Product Specific Information
This antibody detects endogenous levels of USP28 only when phosphorylated at Ser714.
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. Attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity.
Deubiquitination, catalyzed by deubiquitinating enzymes, provides an additional level of regulation and includes four distinct families:
- Ubiquitin C-terminal hydrolases
- Ubiquitin-specific processing proteases (USPs)
- OTU-domain ubiquitin-aldehyde-binding proteins
- Jab1/Pad1/MPN-domain-containing metallo-enzymes
Among these, USPs are the most widespread and conserved across evolution. They release ubiquitin from conjugated proteins and possess catalytic domains with conserved Cys and His boxes, while their variable terminal extensions contribute to substrate recognition, localization, and protein-protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: PA5-106054_USP28_Q96RU2-1_Rabbit.svg, PA5-106054_USP28_Q96RU2-1_Rabbit_PDP.jpeg)
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