Thermo Fisher Scientific Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (10 µg)/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Dog

Published species

Dog

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572935

Product Specific Information

Description: The Canine Fc Receptor Binding Inhibitor can be used to inhibit the non-specific Fc receptor (FcR)-mediated binding of rat monoclonal antibodies used for flow cytometric analysis of canine tissue. FcR are expressed at varying levels in multiple cell lineages including high expression in myeloid cells, granulocytes, and B cells. The biological function of the FcR, including initiation of endocytosis, phagocytosis, and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which rat monoclonal antibodies will bind to canine FcR varies depending on the isotype of the monoclonal antibody.

eBioscience testing indicates that rat IgG2b isotype is blocked by Canine Fc Receptor Binding Inhibitor.

Applications Reported: Canine Fc Receptor Binding Inhibitor Purified has been reported for use in flow cytometric analysis.

To inhibit the non-specific binding of rat monoclonal antibodies add the canine Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with primary antibody.

Applications Tested: Canine Fc Receptor Binding Inhibitor Purified has been pre-titrated and tested by flow cytometric analysis on canine peripheral blood cells. This can be used at 5 µL (10 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.