Thermo Fisher Scientific Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (10 µg)/test
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Product Specifications

Product Specific Information

The Canine Fc Receptor Binding Inhibitor is used to inhibit non-specific Fc receptor (FcR)-mediated binding of rat monoclonal antibodies during flow cytometric analysis of canine tissue.
FcR are expressed in multiple cell lineages, with high levels in myeloid cells, granulocytes, and B cells. Their biological functions—endocytosis, phagocytosis, and antigen presentation—are triggered upon host-immunoglobulin binding.

eBioscience testing indicates that rat IgG2b isotype binding is blocked by the Canine Fc Receptor Binding Inhibitor.

Applications Reported:
Canine Fc Receptor Binding Inhibitor Purified has been reported for use in flow cytometric analysis.

Usage Instructions:
To inhibit non-specific binding of rat monoclonal antibodies, add the inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with the primary antibody.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis on canine peripheral blood cells.
Recommended usage: 5 µL (10 µg) per test.
A test is defined as the antibody amount (µg) that stains a cell sample in a final volume of 100 µL. Cell number may range from 10⁵ to 10⁸ cells/test.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Four classes of Fcγ receptors have been identified: FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16).
They are expressed at varying levels across cell lineages, including high expression in myeloid, granulocyte, and B cells.
FcγR biological functions—endocytosis, phagocytosis, and antigen presentation—are elicited upon host-immunoglobulin binding.
Binding of monoclonal antibodies to FcγRs varies depending on antibody isotype and even among antibodies of the same isotype.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.