Thermo Fisher Scientific Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The human FcγR-binding inhibitor is used to inhibit non-specific Fcγ receptor (FcγR)-mediated binding of mouse monoclonal antibodies during flow cytometric analysis of human tissues.
Four classes of Fcγ receptors have been identified: FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), expressed variably across cell lineages such as myeloid, granulocyte, and B cells. FcγR functions include endocytosis, phagocytosis, and antigen presentation upon host immunoglobulin binding. Binding affinity of mouse monoclonal antibodies to human FcγR depends on antibody isotype and even varies among antibodies of the same isotype.

Applications Reported:
Purified Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis.

Usage Instructions:
To inhibit non-specific binding of mouse monoclonal antibodies, add the human Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with the primary antibody.

Applications Tested:
Pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. Recommended usage is 20 µL per test.
A test corresponds to the antibody amount required to stain a cell sample in 100 µL final volume. Cell number should be empirically determined (10⁵–10⁸ cells/test).

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Four classes of Fcγ receptors (FcγRI/CD64, FcγRII/CD32, FcγRIII/CD16) are expressed at varying levels across multiple cell lineages, including high expression in myeloid, granulocyte, and B cells. Their biological functions—endocytosis, phagocytosis, and antigen presentation—are triggered upon binding to host immunoglobulin. The degree of mouse monoclonal antibody binding to human FcγR varies by isotype and even among antibodies of the same isotype.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.