Thermo Fisher Scientific Nephrin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

ELISA (ELISA)

1 µg/mL

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1019-1241 of human NPHS1 (NP_0046371) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nephrin,uniProtId:O60500-1,ncbiNodeId:9606,antigenRange:1019-1241,antigenLength:1241,antigenImageFileName:PA5-91907_Nephrin_O60500-1_Rabbit.svg,antigenImageFileNamePDP:PA5-91907_Nephrin_O60500-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.68 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806323

Product Specific Information

Positive Samples: Mouse kidney, mouse brain, rat brain; Cellular Location: Cell membrane, Single-pass type I membrane protein

Immunogen sequence: LGDSGLADKG TQLPITTPGL HQPSGEPEDQ LPTEPPSGPS GLPLLPVLFA LGGLLLLSNA SCVGGVLWQR RLRRLAEGIS EKTEAGSEED RVRNEYEESQ WTGERDTQSS TVSTTEAEPY YRSLRDFSPQ LPPTQEEVSY SRGFTGEDED MAFPGHLYDE VERTYPPSGA WGPLYDEVQM GPWDLHWPED TYQDPRGIYD QVAGDLDTLE PDSLPFELRG HLV

Target Information

Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier and it may anchor the podocyte slit diaphragm to the actin cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.