Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (CRM56), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific IL-1 beta Monoclonal Antibody (CRM56), Brilliant Violet 650, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 647
• Brilliant UV 395
• FITC
• Functional Grade
• PE
• Request custom conjugation available

Product Specific Information

The CRM56 antibody reacts with human and baboon interleukin-1 beta (IL-1β), a 17 kDa factor secreted primarily by monocytes. IL-1β influences T-helper cells by inducing IL-2 secretion and receptor expression, and promotes B cell proliferation and immunoglobulin synthesis.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-diluted and validated using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended usage: 5 µL (0.25 µg) per test (100 µL total volume).

Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm and is optimized for cytometers equipped with a violet (405 nm) laser. Ensure compatibility before use.

Staining Panel Considerations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation Compatibility:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact.
Not compatible with methanol-based fixation.

Excitation / Emission / Laser:
407 nm / 649 nm / Violet Laser.

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates and is used under license. Powered by Sirigen™.

Target Information

Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized as a 31 kDa inactive pro-form and cleaved into an active 17 kDa protein via inflammasome activation and caspase-1 processing. IL-1β signals through IL-1RI and IL-1RII receptors, shared with IL-1α. Activity is regulated by IL-1 Receptor Antagonist (IL-1RA). Elevated IL-1β levels are associated with chronic inflammatory conditions, indicating potential therapeutic relevance for IL-1β neutralizing antibodies.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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