Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Brilliant Violet 650, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• eFluor 450
• eFluor 506
• eFluor 660
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The 4S.B3 monoclonal antibody reacts with human interferon-gamma (IFN-γ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic properties.
It is suitable for intracellular staining followed by flow cytometric analysis.
This antibody has been pre-diluted and tested using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test.

Fluorochrome characteristics:
Brilliant Violet™ 650 (BV650) emits at 649 nm and is excited by a 405 nm violet laser. Ensure cytometer compatibility.

Staining considerations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to reduce non-specific interactions.

Fixation and storage:

• Protect from light (photo-sensitive dye).
• Fixed samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C.
• BV650 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 649 nm
Laser: Violet

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

IFN gamma (Interferon gamma, Type II interferon)

• Produced by T-lymphocytes and NK cells in response to antigens and cytokines
• Functions: macrophage activation, antiviral and antiproliferative activity, MHC induction, and immunoglobulin secretion enhancement
• Structure: homodimer of two 146 amino acid subunits (~45 kDa)
• Highly species-specific; human IFN-γ does not cross-react with mouse
• Regulated by IL-2, FGF, EGF (upregulation) and vitamin D3, DMN (downregulation)
• Gene mutations associated with aplastic anemia

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지