Thermo Fisher Scientific IL-6 Monoclonal Antibody (MP5-20F3), Brilliant Violet 650, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• eFluor 450
• FITC
• Functional Grade
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6), a 21–28 kDa cytokine produced by monocytes, fibroblasts, endothelial cells, and other immune cells. It is a neutralizing antibody affecting antigen-specific immune and inflammatory responses and neuronal development.

Applications Reported:
Reported for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested by intracellular staining and flow cytometry using mouse thioglycolate-elicited peritoneal exudate cells with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Recommended use ≤ 0.5 µg/test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test. Optimize antibody titration for best results.
Refer to Flow Protocols for detailed staining procedures.

Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm and is excited by a violet (405 nm) laser. Ensure the cytometer supports this fluorochrome.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light due to photo-induced oxidation sensitivity.

Fixation:
Samples may be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.
Not compatible with methanol-based fixation.

Excitation/Emission/Laser:
407 nm / 649 nm / Violet Laser

Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.™

Target Information

IL-6 encodes a cytokine involved in inflammation and B cell maturation. It acts as an endogenous pyrogen and mediates inflammatory responses via the interleukin 6 receptor alpha. Elevated IL-6 levels are linked to inflammatory diseases such as rheumatoid arthritis, diabetes mellitus susceptibility, and viral infections including COVID-19.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지