Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Violet 650, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.25 µg/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• APC
• Biotin
• Brilliant UV 395 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 711 / 786
• eFluor 450 / 506 / 660
• FITC
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eBio17B7 antibody reacts with mouse and rat IL-17A without recognizing IL-17F. IL-17A is a CD4+ T cell-derived cytokine promoting inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.

Applications Reported

• Intracellular staining followed by flow cytometric analysis.

Applications Tested

• Tested using mouse splenocytes with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
• Recommended use: ≤ 0.25 µg per test (100 µL final volume).
• Optimal antibody concentration should be titrated empirically.

Fluorochrome Information

• Brilliant Violet™ 650 (BV650) emits at 649 nm, excited by a 405 nm violet laser.
• Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions when using multiple polymer dyes.
• Light sensitivity: Protect vial and stained samples from light.
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.
• Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 649 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases.
IL-17A is a 32 kDa disulfide-linked homodimer (136 amino acids) signaling through IL-17R (CDw217). It enhances ICAM-1 expression and stimulates secretion of IL-6, IL-8, G-CSF, and PGE2, supporting proliferation and maturation of hematopoietic progenitors. IL-17A regulates NF-κB and MAPK pathways and enhances COX-2 and nitric oxide production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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