Thermo Fisher Scientific Bcl-xL Monoclonal Antibody (C.85.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:600-1:2400

-

Immunocytochemistry (ICC/IF)

1:200-1:800

-

Flow Cytometry (Flow)

1:200-1:800

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

C.85.1

Immunogen

Synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BCL-XL,uniProtId:Q07817-1,ncbiNodeId:9606,antigenRange:61,antigenLength:233,antigenImageFileName:MA5-15142_BCL-XL_Q07817-1_Rabbit.svg,antigenImageFileNamePDP:MA5-15142_BCL-XL_Q07817-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

144 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10979212

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other Bcl-2 family members.

Target Information

BCL-XL is a part of the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL-XL is located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential and controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.