Thermo Fisher Scientific Triadin Monoclonal Antibody (GE 4.90)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 18 publications 18 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rabbit, Rat

Published species

Mouse, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

GE 4.90

Immunogen

Purified rabbit fast skeletal muscle triadin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2287797

Product Specific Information

MA3-927 detects triadin from mouse, rabbit and rat tissues.

MA3-927 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation and ELISA procedures. By Western blot, this antibody detects a 95 kDa protein representing triadin from rabbit skeletal muscle extracts. Immunohistochemical staining of triadin in rat skeletal muscle with MA3-927 results in striated staining consistent with sarcoplasmic reticulum localization.

The MA3-927 antigen is purified rabbit fast skeletal muscle triadin.

Target Information

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPRs) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyRs), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPRs and RyRs. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.