Thermo Fisher Scientific Triadin Monoclonal Antibody (GE 4.90)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA3-927 detects triadin from mouse, rabbit, and rat tissues.
It has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, and ELISA procedures.
By Western blot, this antibody detects a 95 kDa protein representing triadin from rabbit skeletal muscle extracts.
Immunohistochemical staining of triadin in rat skeletal muscle with MA3-927 results in striated staining consistent with sarcoplasmic reticulum localization.
The MA3-927 antigen is purified rabbit fast skeletal muscle triadin.

Target Information

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad.
At the triad, dihydropyridine receptors (DHPRs) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyRs), the calcium release channels, exist in the membrane of the terminal cisternae of the SR.
It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95 kDa), transmits electrochemical signals to the SR through direct interaction with both DHPRs and RyRs.
Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RyR at the junctional face of the terminal cisternae.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.