Thermo Fisher Scientific ATP1A1 Monoclonal Antibody (9-A5)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1/100

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

1/50

-

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Neutralization (Neu)

-

View 2 publications 2 publications

Inhibition Assays (IA)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

9-A5

Immunogen

Purified rat kidney sodium/potassium ATPase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATP1A1,uniProtId:P06685-1,ncbiNodeId:10114,antigenRange:1-1023,antigenLength:1023,antigenImageFileName:MA3-924_ATP1A1_P06685-1_House_mouse.svg,antigenImageFileNamePDP:MA3-924_ATP1A1_P06685-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2061129

Product Specific Information

MA3-924 detects the alpha 1 sodium/potassium ATPase in human, mouse, canine, rat and avian tissues.

MA3-924 has been successfully used in immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA and immunoprecipitation procedures. Immunohistochemical staining of sodium/potassium ATPase in rat liver with MA3-924 yields a pattern consistent with plasma membrane localization. Immunoprecipitation of sodium/potassium ATPase from canine kidney extracts can be used for affinity purification of the alpha subunit. MA3-924 can also be utilized to inhibit activity of the enzyme.

The MA3-924 antigen is purified rat kidney sodium/potassium ATPase. This antibody recognizes an epitope within an intracellular region at or near Asp-369 of the sodium/potassium ATPase alpha subunit.

Target Information

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.