Thermo Fisher Scientific Calmodulin Monoclonal Antibody (6D4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bacteria, Bovine, Chicken, Human, Rat

Published species

Algae, Cnidarian, Coral, Fruit fly, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6D4

Immunogen

Calmodulin purified from Dictyostelium discoideum. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calmodulin,uniProtId:P0DP23-1,ncbiNodeId:9606,antigenRange:1-149,antigenLength:149,antigenImageFileName:MA3-918_Calmodulin_P0DP23-1_House_mouse.svg,antigenImageFileNamePDP:MA3-918_Calmodulin_P0DP23-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325501

Product Specific Information

MA3-918 detects calmodulin from bovine, chicken, Chlamydomonas, Dictyostelium, and rat samples. This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).

MA3-918 has been successfully used in Western blot, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in Dictyostelium cells with MA3-918 results in staining of the contractile vacuoles.

The MA3-918 antigen is calmodulin purified from Dictyostelium discoideum.

Target Information

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.