Thermo Fisher Scientific SERCA2 ATPase Monoclonal Antibody (2A7-A1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 214 publications 214 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

1/100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:250

View 13 publications 13 publications

Flow Cytometry (Flow)

1:20

-

Immunoprecipitation (IP)

Assay-dependent

View 6 publications 6 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Guinea pig, Human, Mouse, Pig, Rabbit, Rat

Published species

Amphibian, Dog, Fruit fly, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rodent, Sheep, Xenopus

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2A7-A1

Immunogen

Purified canine cardiac sarcoplasmic reticulum vesicles. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SERCA2 ATPase,uniProtId:O46674-1,ncbiNodeId:9615,antigenRange:1-997,antigenLength:997,antigenImageFileName:MA3-919_SERCA2_ATPase_O46674-1_House_mouse.svg,antigenImageFileNamePDP:MA3-919_SERCA2_ATPase_O46674-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325502

Product Specific Information

MA3-919 detects sarcoplasmic or endoplasmic reticulum calcium (SERCA) 2 ATPase from canine, porcine, human, mouse, rabbit and rat tissues. MA3-919 recognizes the SERCA2a and SERCA2b isoforms identically. However, the antibody does not recognize the skeletal muscle (SERCA1) or housekeeping isoforms (SERCA3).

MA3-919 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase in rat cardiac tissue.

The MA3-919 antigen is purified canine cardiac sarcoplasmic reticulum vesicles.

Target Information

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.